Interaction of the restriction endonuclease ScaI with its substrates.

نویسندگان

  • K Kita
  • N Hiraoka
  • F Kimizuka
  • A Obayashi
  • H Kojima
  • H Takahashi
  • H Saito
چکیده

The kinetic constants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa- greater than deca-, dodeca-, hexadeca- greater than pBR322). The results were discussed with respect to the tertiary structure of substrate.

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عنوان ژورنال:
  • Nucleic acids research

دوره 13 19  شماره 

صفحات  -

تاریخ انتشار 1985